Figure 9.

The TrHBMECs and HMVECs attach to a laminin α4-fragment in an αvβ3-integrin–dependent manner. (A) TrHBMECs were plated in a 96-well plate in uncoated wells, wells coated with the α4 laminin subunit fragment, laminin-1 (LN), or fibronectin (FN) in the presence or absence of antibody LM609 against αvβ3, antibody 2A3 against the α4 laminin subunit, P4C10 against the β1-integrin or control IgG. TrHBMECs attach efficiently to the fragment, laminin-1, and to fibronectin within 90 min after plating. Antibodies 2A3 and LM609 inhibit cell attachment to the α4 laminin fragment, whereas the same antibodies do not inhibit cell attachment to fibronectin. P4C10 has a minor inhibitory effect on attachment of TrHBMECs to the α4 chain fragment, whereas the same antibody significantly inhibits attachment to laminin-1. (B) HMVECs were plated onto uncoated wells or wells coated with the recombinant fragment in the presence or absence of growth factors. The attachment of cells was assayed after 120 min. Attachment of HMVECs to the α4 laminin fragment is stimulated by growth factors but can be inhibited by antibody 2A3 and LM609.