Table I. Oligonucleotide probes used in EMSAs.
| Probe | Nucleotide sequencea | Nt position/reference |
|---|---|---|
| Eα 5′-Ets | 5′-GAAGTAGAACAGGAAATGGAAA-3′ | Eα 51–72 |
| Eα 5′-Ik | 5′-GAAAAAGTTTCCCACTTCCCTCCAG-3′ | Eα 69–93 |
| Eα ATF/CREB | 5′-TCCATGACGTCACGGCTGCT-3′ | Eα 121–140 |
| Eα LEF/CBF/Ets | 5′-ACAGGTCCCCCTTTGAAGCTCTCCCGCAGAAGCCACATCCTCTGGAA-3′ | Eα 152–199 |
| Eα CBF-Ets | 5′-TCTCCCGCAGAAGCCACATCCTCTGGAA-3′ | Eα 161–199 |
| EBS-a | 5′-GATAACAGGAAGTGGTTGTA-3′ | Bosselut et al. (1993) |
| EBS-z | 5′-GATAACACCAAGTGGTTGTA-3′ | Bosselut et al. (1993) |
| IK-BS4 | 5′-TCAGCTTTTGGGAATGTATTCCCTGTCA-3′ | Molnár and Georgopoulos (1994) |
| IK-BS5 | 5′-TCAGCTTTTGAGAATACCCTGTCA-3′ | Molnár and Georgopoulos (1994) |
| CREB-BS | 5′-CGCCTTGAATGACGTCAAGGC-3′ | Plet et al. (1997) |
aSequences are in the 5′ to 3′ orientation; only the top strand is indicated. Transcription factor binding motifs are underlined.