Sequential C-banding (a) and genomic in situ hybridization (b) on a 20 chromosome C0 androgenic triticale (XTriticosecale Wittmack) plant, nullisomic for rye chromosome 5R. Rye chromatin labeled green; wheat chromatin is red. c C-banded 28 chromosome C0 plant with 21 wheat and 7 rye chromosomes. Wheat chromosomes are: 1A′, 2A′, 3A′, 4A″, 5A″, 6A″, 7A′, 1B″, 2B″, 3B′, 4B″, 5B′, 6B′, 7B″ where ′ and ″ denote monosomics and disomics, respectively. d Genomic in situ hybridization with rye genomic probe on a 40 chromosome C1 androgenic triticale plant. Rye chromatin labeled green; wheat chromatin counterstained red: 26 wheat chromosomes, 6 pairs of rye chromosomes and one pair of non-centric wheat-rye translocation (arrowed). The rye chromosome in the translocation is 6R; the wheat segment remains to be unidentified