Figure 1.

Nfasc Is Not Required for AIS Assembly

(A) Immunofluorescence analysis of Calbindin-positive Purkinje cells in sagittal cryostat sections of cerebella from P6 wild-type littermates and Nfasc^−/− mice shows no difference in the localization of voltage-gated sodium (Na_v) channels, AnkyrinG, and βIV-Spectrin at the AIS. However, NrCAM fails to localize at the AIS in the mutant (arrowheads point to AIS location). Scale bar, 10 μm.

(B) Immunofluorescence of Calbindin-positive Purkinje cells in sagittal cryostat sections from P6 Nfasc^+/+/Nfasc186 and Nfasc^−/−/Nfasc186 mice shows that Nfasc186Flag is correctly targeted to the AIS and that its expression on a Nfasc null background can restore NrCAM to the AIS. Scale bar, 10 μm.

See also Figure S1.