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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Vaccine. 2009 Jan 20;27(10):1589–1595. doi: 10.1016/j.vaccine.2008.12.058

Fig. 5.

Fig. 5

Inhibition of VSC production and biofilm formation by anti-F. nucleatum serum. F. nucleatum (4 × 109 CFU) was pre-incubated with 2.5% (v/v) anti-F. nucleatum (Anti-N) or anti-S. mutans (Anti-M) sera without activated complements for 2 h. Afterwards, serum-bound F. nucleatum was cultured on a 6-well for additional 36 h under anaerobic conditions. VSC production (A) and biofilm formation (B) were detected by lead acetate-containing agar plates and crystal violet staining, respectively, as described in Section 2. Bars = 1.5 cm.