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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Cancer Res. 2011 Jan 21;71(5):1730–1741. doi: 10.1158/0008-5472.CAN-10-1432

Figure 4. Arg is required for Src-mediated stimulation of actin barbed end formation in invadopodia.

Figure 4

(A) Representative images of MDA-MB-231 cells stably expressing Src-TagRFP (a-d) or cells stably expressing Arg-YFP (e-h), treated with control siRNA (a,b,e,f), Arg siRNA (c,d), or Src siRNA (g,h). Cells were either left untreated (a,c,e,g), or stimulated with EGF for 3 min. (b,d,f,h), fixed and stained for biotin-actin and Arp2 as an invadopodium marker. (B) Quantification of barbed ends as measured by the average actin intensity at stimulated invadopodia in response to EGF (all conditions n=200). The difference between all 3 minute stimulation values are statistically significant as measured by individual Post-Hoc student’s t-test (p<0.01). Scale bars=5μm. (C) Representative images of cells knocked down for endogenous Arg and re-expressing YFP, Arg-YFP, or mutant Arg Y439F-YFP. Scale bars=5μm. (D) Cells were stimulated and treated as above, and actin barbed ends were quantified as in B. YFP: n=127(0min.)/n=275(3min.), Arg-YFP: n=308(0min.)/n=209(3min.), ArgKI-YFP: n=196(0min.)/n=181(3min.), STI-571: n=289(0min.)/n=415(3min.).