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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: J Immunol. 2011 Jan 21;186(4):2033–2041. doi: 10.4049/jimmunol.1003015

Figure 4. Memory CD8+ T cells generated under conditions of limited antigen results in similar levels of cytokine production and similar phenotypic profiles.

Figure 4

Mice infected with LM-OVA after OT-I transfer were either treated with ampicillin or left untreated, sacrificed 30 days post infection and splenocytes were harvested for cytokine production assays and assessment of phenotypic expression markers. A-C) Splenocytes from LM-OVA-infected OT-I recipients were restimulated in vitro with SIINFEKL peptide for 4 hours, and analyzed for the presence of intracellular IFN-γ, TNF, and IL-2. Untreated and ampicillin-treated mice showed similar levels of production of IFN-γ, IL-2, and TNF. Data shown in A are representatives of two independent experiments, where n=5 animals per group per experiment. Data in B show summary data for both independent experiments. D) CD8+Thy1.1+ T cells from untreated or ampicillin-treated recipients were analyzed at day 30 post-infected for expression of CD62L, CD127, KLRG-1. CD28, and CD25. Mean fluorescence intensities of Thy1.1+ CD8+ T cells of two separate experiments are shown, for a total of five- 10 mice per group.