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. 2001 Jan;12(1):115–128. doi: 10.1091/mbc.12.1.115

Figure 5.

Figure 5

Analysis of the Spp1 mutant proteins. (A) Levels of Spp1 protein in wild-type and spp1 mutants at the permissive and the restrictive temperature. Total cell extracts prepared from cells grown for 5 h at 25 or 36°C were prepared and analyzed by immunoblotting as described in MATERIALS AND METHODS. (B) Analysis of the association of mutant-Spp1 proteins with the Polα immunocomplex. Polα immunocomplex was analyzed by immunoblotting with antibodies against Polα, Spp2, and Spp1 as described in MATERIALS AND METHODS.