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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Leuk Lymphoma. 2010 Apr;51(4):702–714. doi: 10.3109/10428191003646697

Figure 1.

Figure 1

Calcium concentration as an indicator of osteoclastic activity ex vivo. (A) Calcium concentrations in culture media of calvaria treated with PTH (10 nM) or IL-1 (2 ng/mL) for 6 days with (Z) or without (C) overnight pretreatment with zoledronic acid (Z). The symbol ‘*’ indicates significant differences when compared to C-cont (control). Calcium concentration in medium without calvaria is also shown. (B) TRAP staining on whole-mounted calvaria. Activated osteoclasts were stained dark red.