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. 2010 Sep 21;22(3):293–301. doi: 10.1089/hum.2010.069

FIG. 1.

FIG. 1.

(A) Schematic of the expression cassette packaged in our Ad-GLA-FLAG gene transfer vector. The CMV promoter drives expression of GLA fused to a C-terminal FLAG sequence followed by a stop codon. Downstream from the stop codon, an internal ribosomal entry site (IRES) initiates expression of green fluorescent protein (GFP). (B) GLA assay of cellular lysates from AD-293 cells 24 hr after infection with 100 viral particles of Ad-GLA-FLAG (mock-infected cells are negative controls). (C) GLA assay of anti-FLAG column eluate at two different sample concentrations (5 or 50 ml of eluate in 400 ml of citrate buffer with 20 ml of substrate; note: protein concentration of eluate was not determined). (D) GLA assay of salivary gland tissue homogenates 48 hr after delivery of Ad-GLA-FLAG to the right submandibular gland. Glands from animals receiving a saline infusion served as negative controls. Error bars are ± SEM.