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. 2011 Apr;162(7):1564–1576. doi: 10.1111/j.1476-5381.2010.01158.x

Figure 9.

Figure 9

(A) The relative abundance of mRNA for the adipocyte fatty acid-binding protein (A-FABP) gene in human microvascular endothelial cells (HMECs) was quantified by real-time PCR analysis and normalized against 18S RNA. (B, C) The protein levels of A-FABP, phosphorylated (p-eNOS) and total eNOS (T-eNOS) with or without stimulation with palmitate (PA) and insulin (Ins) was analyzed by Western blotting. (D) NO production measured by cGMP assay using the same condition as in panel C (n= 5–6). (E) Protein levels of p-eNOS (ser1177) and total eNOS with or without A-FABP inhibitor treatment in the presence of palmitate (0.1 to 0.3 mM; PA) or/and insulin (100 nM; Ins) in HMECs. (F) NO production measured by cGMP assay using the same conditions as in panel E (n= 4–5). The bar graphs show the data as means ± SEM *P < 0.05; **P < 0.01.