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Effect of latrunculin-A on GLUT4-eGFP and EEA1. 3T3-L1 adipocytes expressing GLUT4-eGFP (control) were exposed to Lat-A for 20 min, fixed, permeabilized, and stained with a polyclonal antibody raised to EEA1. Each panel is the projection in a single two-dimensional plane of 30 optical sections through the juxtanuclear region of the cell. The arrows on the top right panel point to vacuoles containing GLUT4-eGFP. Bar, 3 μm.
