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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Matrix Biol. 2010 Nov 3;30(2):145–153. doi: 10.1016/j.matbio.2010.10.007

Fig. 1.

Fig. 1

Time-course of aggrecanolysis in response to cytokine stimulation. Bovine articular cartilage explants were cultured for 19 days in the presence of IL-1β/OSM and tissue was collected at different time points and analyzed by SDS/PAGE and immunoblotting. Aggrecan degradation in the IGD was monitored using an anti-aggrecan G1 antibody (anti-G1) or anti-neoepitope antibodies directed against the new C-terminus generated by aggrecanase cleavage at the Glu373–374Ala site in the IGD (anti-NITEGE) and MMP cleavage at the Ser341–342Phe site in the IGD (anti-DIPES). Migration positions of aggrecan products generated by aggrecanase (G1 Agg) or MMP (G1 MMP) action are indicated on the right and the migration positions of molecular weight markers are indicated on the left. The days of culture are indicated below the immunoblots.