Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Matrix Biol. 2010 Nov 3;30(2):145–153. doi: 10.1016/j.matbio.2010.10.007

Fig. 8.

Fig. 8

Comparison of the ability of ADAMTS5 and MMP12 to cleave within the aggrecan IGD. Bovine aggrecan was digested with ten-fold dilutions of 10-nM ADAMTS5 and 10-nM MMP12. Digested samples were then analyzed by SDS/PAGE and immunoblotting using an anti-aggrecan G1 antibody (anti-G1). Migration positions of aggrecan products generated by aggrecanase (G1 Agg) or MMP (G1 MMP) action are indicated on the right and the migration positions of molecular weight markers are indicated on the left. The concentrations of ADAMTS5 and MMP12 used for aggrecan digestion are indicated below the immunoblot. The band at ~26 kDa represents biotinylated SBTI used as a loading control.