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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2010 Dec 30;50(4):652–661. doi: 10.1016/j.yjmcc.2010.12.015

Fig. 1.

Fig. 1

Interaction of Abro1 with THAP5 in yeast and in human cardiomyocytes. (A) Yeast colonies were transformed with plasmids encoding the indicated baits. Full-length Abro1 was used as prey. Blue color results from a positive protein-protein interaction. (B) THAP5 antibody was used to precipitate the endogenous protein as well as any THAP5 associated proteins in human heart lysate. The precipitated complex was resolved by SDS-PAGE, and the presence of Abro1 in the complex was monitored using a specific antibody. Lane 3 shows that Abro1 co-precipitated with THAP5, and there was no interaction when pre-immune serum was used (lane 2). Lane 1 shows total heart lysate. The “*” indicates a non-specific protein band seen in all our co-IP experiments.