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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2010 Dec 30;50(4):652–661. doi: 10.1016/j.yjmcc.2010.12.015

Fig. 3.

Fig. 3

Subcellular localization of Abro1 protein and mRNA expression in human, mouse, and rat cardiomyocytes. (A) Adult human cardiomyocytes were stained with Abro1 antibody (panel A) as well as the sarcomeric specific marker anti-α-actinin (panel B). Panel C shows DAPI staining of the same cells and panel D shows the merged images including differential interference contrast (DIC). Abro1 is predominantly found in the cytoplasm of the cardiomyocytes. (B) Abro1 mRNA expression in cardiomyocytes was detected by RT-PCR. PCR products were obtained using species-specific PCR primers and analyzed on a 1.5% agarose gel. The first and last lanes represent DNA size markers. “Abro” indicates the PCR product obtained with human Abro1-specific primers (560bp), or mouse and rat specific primers (730bp). Abro1 gene expression was normalized to GAPDH (Gh) (570bp).