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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2010 Dec 30;50(4):652–661. doi: 10.1016/j.yjmcc.2010.12.015

Fig. 8.

Fig. 8

MI/R leads to a decrease in K63-linked polyubiquitination of specific target proteins. Western blot analysis of heart extracts prepared from mouse hearts that underwent MI/R (lanes 3 and 4) or sham operated (lanes 1 and 2). A specific antibody that recognizes K63-linked ubiquitinated proteins was used as described in the experimental procedures. The bottom panel shows the Ub-K63/GAPDH ratio of the two major K63-linked ubiquitinated polypeptides found in the heart extracts. The K63-linked ubiquitination on these proteins was significantly reduced in the heart extracts from mice that underwent MI/R. GAPDH antibody was used to verify equal loading per lane. The bottom panel shows Ub-K63/GAPDH ratio that was calculated after densitometry analysis of the respective upper and lower bands.