Table 1. Effect of NH₄Cl loading on blood and urine electrolyte and acid-base composition in wild-type and TASK^-/- mice.

	WT		KO
	Pre	Post	Pre	Post
Metabolic Parameter
Body Wt (g)	25.8±0.5	25.2±0.2	24.9±1.0	25.0±0.6
NH4Cl intake (g/day)	N/A	0.35±0.02	N/A	0.35±0.02
UNa (mmol/mg cre)	0.30±0.04	0.89±0.09*	0.41±0.05	1.14±0.03*
UK (mmol/mg cre)	1.01±0.14	2.36±0.19*	1.35±0.16	3.07±0.10*
Blood acid-base
pH	7.19±0.02	7.06±0.03*	7.21±0.02	7.10±0.02*
HCO3 (mmol/L)	21.57±1.72	16.11±1.45*	23.77±0.93	17.01±0.73*
PCO2 (mmHg)	57.86±6.60	56.60±3.44	59.48±3.30	55.16±3.33
PO2 (mmHg)	55.80±3.12	51.00±2.92	48.80±3.18	56.00±3.51
BE (mmol/L)	-6.86±1.34	-14.29±1.86*	-4.78±0.62	-12.67±0.91*
Blood parameters
Cl (mmol/L)	116.33±0.88	123.67±2.33	117.83±1.74	120.83±2.33
Na (mmol/L)	144.71±1.39	154.14±1.20*	149.00±0.90	159.33±3.35*
K (mmol/L)	5.8±0.39	4.57±0.23*	4.10±0.17	3.56±0.17
iCa (mmol/L)	1.21±0.05	1.32±0.04	1.29±0.04	1.30±0.04
Hct (%PCV)	39.86±1.97	35.43±1.34*	42.63±0.68	38.25±0.77*
Hb (g/dL)	13.54±0.67	12.06±0.46*	14.50±0.23	13.06±0.24*
BUN (mg/dL)	28.50±2.72	29.33±1.86	28.71±1.69	24.00±1.86

Values are means ± SE of samples obtained during a 3 day pre-NH₄Cl control period and during days 4-6 of NH₄Cl administration. Comparisons of group means were made using a repeated measures ANOVA with Bonferroni post hoc tesing.

^*indicates statistical significance vs. before acid-load (p<0.05). Urinary K and Na were measured using flame photometry and renin concentration was measured with RIA. All other blood measures were determined with an iSTAT hand-held blood analyzer. PCO₂, partial pressure of CO₂; PO₂, partial pressure of O₂; BE, base excess; iCa, ionized Ca; Hct, hematocrit; Hb, hemoglobin; BUN, blood urea nitrogen.