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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Metab Eng. 2010 Dec 3;13(2):241–251. doi: 10.1016/j.ymben.2010.11.002

Figure 1. Single-cell Fluorescent Detection of IgG Expression.

Figure 1

(A) General scheme of E. coli mutagenesis and isolation of clones displaying increased IgG expression using periplasmic expression with cytometric screening (PECS). (B) Fluorescence histograms of E. coli JUDE-1 cells over-expressing either 26.10 IgG or YMF10 IgG (anti-PA antibody (Mazor et al., 2007)); negative control) with different combinations of leader peptides for heavy and light chain periplasmic export. Statistics correspond to a population of 10,000 cells. M: mean fluorescence intensity. (C) Growth of E. coli JUDE-1 cells over-expressing 26.10 IgG antibody with different leader peptides for heavy and light chain periplasmic export. OD600: optical density at 600 nm, Plac-pelB-pelB: lac promoter with pelB leader peptide for light and heavy chain periplasmic export, Plac-pelB-dsbA: lac promoter with pelB leader peptide for periplasmic export of light chain and dsbA leader peptide for periplasmic export of heavy chain.