Table 1.
Conservation of JHAMT residues critical for interactions with the substrates
| A. aegypti | Q 14 |
D 41 |
D 69 |
V 70 |
I 95 |
W 120 |
L 146 |
I 151 |
I 154 |
Y 155 |
L 158 |
S 176 |
Y 178 |
V 221 |
V 224 |
Activity |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A. gambiae (*) | = | = | = | I | = | = | = | = | = | = | = | = | = | = | = | Not tested |
| D. melanogaster | = | = | = | I | = | = | = | V | V | = | = | = | L | = | I | Yes for FA and JHA |
| B. mori | = | = | = | I | = | = | = | = | V | = | = | = | = | = | I | Yes for FA and JHA |
| S. ricini | = | = | = | I | = | = | I | = | V | = | = | = | = | = | = | Yes for JHA |
| S. litura | = | = | = | I | = | = | V | V | L | = | = | = | = | L | I | Not tested |
| H. armigera | = | = | = | I | = | = | V | V | V | = | = | = | = | L | I | Not tested |
| T. castaneum | = | = | = | I | = | = | = | = | = | = | M | = | = | = | = | Yes for FA and JHA |
| A. mellifera | = | = | = | I | = | = | = | G | V | = | = | P | F | I | I | Not tested |
| N. vitripennis | = | = | = | = | = | = | I | G | S | = | = | P | F | = | = | Not tested |
| A. pisum | = | = | = | K | = | = | = | L | M | = | = | Q | S | = | = | Not tested |
| R. prolixus | = | = | = | I | = | = | = | L | Y | = | F | ? | ? | I | = | Not tested |
=: Identical amino acid. Letters show conserved substitutions. Underlined bold letters are semi-conserved substitutions. Italic bold letters show non-conserved substitutions. Shaded boxes indicate substitutions not present in any of the three species whose activities have been experimentally tested (these three species are in bold).
? : Residues not known.
*
C. pipiens has identical substitutions than A. gambiae.