Skip to main content
. Author manuscript; available in PMC: 2011 Aug 24.
Published in final edited form as: Nature. 2011 Feb 24;470(7335):543–547. doi: 10.1038/nature09737

Figure 3. Immunization with nanoparticles containing MPL+R837 induces persistent GCs and long lived antibody forming cells in draining lymph nodes.

Figure 3

a) C57BL/6 mice were immunized with OVA encapsulated in nanoparticles with MPL+R837 plus antigen. 4 weeks post primary immunization draining lymph nodes were excised, tissue sections prepared and stained for GCs (GL-7 red, B220 blue and IgG green). Images are representative of 2 independent experiments with draining lymph nodes obtained from at 2–3 mice per treatment condition per experiment. b) GCs were counted in LN sections at the time points indicated and represented as mean ± s.e.m from 4–6 draining lymph nodes from n=2–3 mice/treatment group. c) ELISPOT assay. Combination of TLR4 and TLR7 ligands has no effect on the short lived ASCs, but stimulates long lived ASCs that persist for ~1.5 years. Graph represents average spots per 1 × 106 total lymph node cells ± s.e.m from duplicate cultures per treatment group. Data is representative of at least 2–3 independent experiments per time point indicated.