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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Cancer Res. 2011 Jan 6;71(5):1658–1668. doi: 10.1158/0008-5472.CAN-10-1899

Figure 3. Effects of EGF and calcium on the activity of ERα in COS-1 cells.

Figure 3

A,B. COS-1 cells were transiently transfected with wt ERα, truncated ERα mutants HE15 and HEG19, an ERE-CAT reporter or GR and an MMTV-CAT reporter andβ-gal and treated for 24 hours with estradiol (1nM), EGF (150ng/ml), and calcium (1mM) in the presence and absence of the antiestrogen ICI-182,780 (500 nM) and the PLC inhibitor U73122 (2μM). The amount of CAT activity was measured and normalized to the amount of β-galactosidase activity. The results are expressed as percent control (mean±SD; three independent experiments). *, p < 0.05; **; p < 0.005; ***, p < 0.0005. a, compared to control; b, compared to treatment.

C. Schematic representation of ERα mutants.