Skip to main content
. Author manuscript; available in PMC: 2012 Feb 17.
Published in final edited form as: Cell Host Microbe. 2011 Feb 17;9(2):147–157. doi: 10.1016/j.chom.2011.01.005

Figure 3. RAB-5 and RAB-11 are required for defense against Cry5B PFT.

Figure 3

(A) rrf-3(pk1426) animals after rab-5 or rab-11.1 RNAi are qualitatively hypersensitive to a low (10%) dose of E. coli-expressed Cry5B PFT after 48 hr exposure. Scale bar: 0.5 mm. (B) VP303 animals grown in liquid on rab-5, rab-11.1, or sek-1 RNAi bacteria show significantly decreased survival rates on 15 μg/mL purified Cry5B, whereas pgp-1 and empty vector (L4440) controls do not. (C) In simultaneously performed assays, RNAi treatments did not cause significant hypersensitivity to CuSO4. (B) and (C) show means of at least three independent experiments. Statistics indicate difference between toxin treatment and its accompanying no-toxin control for each RNAi treatment. Error bars are standard error of the mean.