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. Author manuscript; available in PMC: 2012 Feb 17.
Published in final edited form as: Cell Host Microbe. 2011 Feb 17;9(2):147–157. doi: 10.1016/j.chom.2011.01.005

Figure 5. RAB-5 and RAB-11 are required for removal of PFT from the plasma membrane.

Figure 5

(A) PI is restricted to the intestinal lumen when fed to C. elegans. PGP-1::GFP marks the apical boundary of the intestinal cells. Scale bar: 25 μm. (B) PI stains the cytosol of the intestinal cells when animals are simultaneously exposed to purified Cry5B PFT for 2 hr. Scale as (A). (C) Fractions of population showing cytosolic PI staining after 0.5 or 24 hr recovery after a 15 minute pulse with E. coli-expressed Cry5B. After 0.5 hr recovery all RNAi treatments resulted in statistically equal fractions of animals with compromised integrity (internalized PI) of the intestinal cells. The majority of control (L4440) animals and animals on pgp-1 RNAi have repaired their intestinal cells after 24 hr recovery. RNAi against rab-5 or rab-11.1 causes significantly impaired repair after 24 hr. Means of at least three independent experiments. Error bars are standard error of the mean.