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. Author manuscript; available in PMC: 2012 Feb 2.
Published in final edited form as: Cell Metab. 2011 Feb 2;13(2):160–169. doi: 10.1016/j.cmet.2011.01.003

Figure 1. The unfolded protein response is activated in skeletal muscle after exercise.

Figure 1

Total RNA and protein lysates from quadriceps were isolated from C57/Bl6 wild-type male mice 5 hrs post one bout treadmill running (n=6) or sedentary control (n=6) as described in experimental procedures. A. Total RNA was analyzed by realtime RT-PCR; Data represented means±SEM. *** indicates p <0.005 vs. control, *, p <0.05. Similar results were observed in more than three independent experiments. B. Protein lysates were probed by immunoblot as indicated, with total eIF2α as a loading control. The first two lanes are positive controls using lysates from primary myotubes treated with 100nM thapsigargin or vehicle for 8hrs. C. Exercise training leads to adaptation in skeletal muscle. Untrained mice (naïve) or trained mice (trained: five sessions of 1hr treadmill run/wk for 4weeks), together with age-matched sedentary control mice were sacrificed 5 hrs after one bout equal distance treadmill running. N=6 animals per group. Total RNA from quadriceps was isolated and analyzed by realtime RT-PCR with CytC as a positive control; Data represented means±SEM. *** indicates p <0.005 trained vs. naïve, **, p <0.01, *, p <0.05. ### indicates p <0.005, rest vs. trained : run, ##, p <0.01, #, p <0.05. Statistical comparison between rest vs. naïve : run is similar to Figure 1A, and not shown in this panel for visual clarity.