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. Author manuscript; available in PMC: 2012 Feb 2.
Published in final edited form as: Cell Metab. 2011 Feb 2;13(2):160–169. doi: 10.1016/j.cmet.2011.01.003

Figure 4. Coactivation of ATF6α by PGC-1α.

Figure 4

A. C2C12 myoblasts were transfected with expression plasmids for XBP-1 coded by spliced form of Xbp-1 mRNA (XBP-1s), cleaved form of ATF6α (ATF6α-N), ATF4 or expression vector control, respectively, together with expression plasmid for PGC-1α, or vector and rat BiP promoter-luciferase construct. The cells were subsequently differentiated for 2 days and harvested and luciferase activity was measured, normalizing against renilla. Data represent means±SD from biological triplicates. B. Coimmunoprecipitation of ATF6α and PGC-1α. Cultured COS cells were transfected with plasmids as indicated. Total lysates from transfected cells were subjected to immunoprecipitation using beads specific for Flag tag. Both lysates and precipitates were analyzed by immunoblotting with antibodies specific for the Flag and HA epitope tag. (C and D) Total RNA from ATF6α+/+ and −/− primary myotubes (C) and IRE1α+/+ and −/− primary myotubes (D) infected with adenovirus expressing PGC-1α or GFP as a control was assayed by realtime RT-PCR for UPR markers expression. Data represent means±SD from biological triplicates.