FIG. 1.

Genetic map of the Tn4555 circular form. Genes are shown by the block arrows, and those genes used in this study are hatched; oriT and the transposon termini (attTn) are indicated by black symbols. DNA fragments used for the construction of the pFD660 family of plasmids and their mobA-oriT derivatives are shown by gray blocks inside the circular map. The location of the 485-bp PCR amplicon used to detect the circular form is shown by the hatched box, and the region of Tn4555-NBU1 homology is indicated by the gray box outside of the circular map.