FIG. 3.

Int but not TnpA is required for Tn4555 excision. Shown are PCR excision assays showing agarose gels of PCRs using the attTn primers as described in Materials and Methods. Standard, 1-kb ladder (InvitroGen, Carlsbad, Calif.); +control, BT5482::341 containing Tn4555 induced with TET; −control, BT5482::341 containing Tn4555 not induced with TET. PCRs were from cultures either induced with 1 μg of tetracycline (+Tc) or not induced (−Tc). Lanes marked with an asterisk are from strains that did not contain CTn341. Arrowheads on the sides of the panels indicate the location of the 506-bp marker which aligns close to the expected PCR product.