TABLE 1.
Substrate | Sp act (U/mg of protein) |
---|---|
6-Biopteridin | <0.05 |
PtH2 | <0.05 |
qPtH2 | 26.2 ± 2.7 |
Potassium ferricyanide | <0.05 |
FH2 | 5.2 ± 1.1 |
Activity was measured at 35°C in 50 mM potassium phosphate buffer (pH 7.5) in the presence of 0.1 mM NADPH and 0.1 mM pteridine.