Skip to main content
. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Chromosoma. 2010 Aug 12;120(1):61–71. doi: 10.1007/s00412-010-0290-9

Fig. 2. Nuclear positions of t(12;15) loci are similar to those on unrearranged chromosomes.

Fig. 2

(a) Relative radial positions of single BACs from Mmu12 and Mmu15 were determined by measuring the distance from the nuclear center to the locus (rx) and normalizing to the length of the total nuclear radius (rn), as depicted. Thus 0 represents the center of the nucleus and 100 the outer nuclear edge. Relative radial positions were measured for Mmu12 sequences in BAC 367G12 and Mmu15 sequences in BAC 200D20 on both unrearranged chromosomes (Mmu12 and Mmu15) and t(12;15) (TL12 and TL15). BACs were co-hybridized to the same cells by two color FISH (Fig. 1b–d). t(12;15) was discriminated from un-rearranged chromosomes by the close positioning of the two BACs (< 1.5 μm). (b) Cumulative distributions of relative radial positions of indicated loci in all three tumors (LP-A (N = 85), LP-B (N = 87), AP-C (N = 96)) and normal B6 Pro-B cells (N = 96). Horizontal grey bars indicate means, boxes represent median and quartile values, and whiskers indicate the remaining points in the distribution. Indicated P-values were derived from Mann-Whitney U tests.