TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | Host for cloning vectors | GIBCO |
| S. typhimurium | ||
| TR6579 | Host for cloning vectors | J. R. Roth |
| TT22236 | LT2/pTP223 | J. R. Roth |
| RMC25 | LT2 dxs::MVA operon | This work |
| RMC26 | LT2 dxs::MVA operon | This work |
| RMC28 | RMC26/pTP223 | This work |
| CR4 | RMC26 ispD::Cam | This work |
| TE3461 | Mud-cam source | J. R. Roth |
| CR5 | RMC26 srlE::Cam | This work |
| CR24 | LT2 srlE::Cam | This work |
| CR29 | CR5 pBAD/srlE | This work |
| RMC78 | RMC26 srlA::Cam | This work |
| RMC79 | RMC26 srlB::Cam | This work |
| Plasmids | ||
| pBAD/gIII | Source of araC and PBAD | Invitrogen |
| pGEM-T Easy | TA cloning vector; Ampr | Promega |
| pGEM-T | TA cloning vector; Ampr | Promega |
| pTP223 | λ-Red genes | J. R. Roth |
| pFCO1 | ERG genes inserted in pBluescript SK+ | 20 |
| pUC4K | Contains Kanr cassette | Pharmacia |
| pFCO1/kan | pFCO1 with Kanr cassettes from pUC4K | This work |
| pCAT53-3 | MVA operon | This work |
| pRMC13 | Serovar Typhimurium dxs in pGEM-T | This work |
| pRMC14 | Plasmid-encoded dxs::MVA operon | This work |
| pBAD/myc-his A | Arabinose-induced expression vector; Ampr | Invitrogen |
| pBAD/srlE | srlE cloned into pBAD/myc-his A | This work |