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Modification of the CgtAC C terminus affects function. Serial dilutions of cultured JM1108 cells containing pMR20 plasmids expressing cgtAC, no protein (None), cgtAC-3HA, cgtAC1-347, or cgtAC1-347-3HA were spotted onto PYE+Xyl and PYE+Glu plates. The plates were incubated at 30°C for 2 days.
