Skip to main content
. 2008 Aug 18;1(5):403–415. doi: 10.1111/j.1751-7915.2008.00041.x

Figure 6.

Figure 6

Intracellular localization and activity of Tat‐targeted MBP–scFv fusions.
A. Western blot analysis of periplasmic (per) and cytoplasmic (cyt) fractions from HS3018 cells expressing ssTorA–MBP‐scFv13 and ssTorA–MBP–scFv13.R4. Western blots were probed with anti‐c‐myc primary antibody.
B. In vitroβ‐galactosidase activity of whole‐cell lysates from E. coli959 cells incubated with purified periplasmic scFv13.R4 targeted through either Sec (ssMBP) or Tat (ssTorA) as an unfused protein (−) or as a fusion to MBP (+). Each sample was assayed in triplicate and data represent the average of three replicate purification and activity experiments.