Table 3.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant genotype or features | Reference or source |
|---|---|---|
| MC4100 | F‐ araD139Δ(argF‐lac)U169 flbB5301 deoC1 ptsF25 relA1 rbsR22 rpsL150 thiA | Laboratory stock |
| B1LK0 | MC4100 ΔtatC | Bogsch et al. (1998) |
| HS3018 | MC4100 malT(Con)‐1ΔmalE444 | Shuman (1982) |
| HS3018 ΔtatC | HS3018 tatC::Spec | Tullman‐Ercek et al. (2007) |
| DHB4 | F′lacIqpro/λ‐ ΔlacX74 galE galK thi rpsL phoRΔphoA(PvuII) ΔmalF3 | Laboratory stock |
| FÅ113 | DHB4 gor522. . . mini‐Tn10Tet trxB::Kan ahpC* | Bessette et al. (1999) |
| 959 | AMEF lacZgene | Messer and Melchers (1978) |
| pTrc99A | trcpromoter, ColE1 ori, Ampr | Amersham Biosciences |
| pMBP | Native MBP in pTrc99A | This study |
| pssTorA(+10)–MBP | ssTorA plus residues 1–10 of mature TorA fused to Δ(1–26)MBP in pTrc99A | This study |
| pssTorA(+6)–MBP | ssTorA plus residues 1–6 of mature TorA fused to Δ(1–26)MBP in pTrc99A | This study |
| pssTorA(+0)–MBP | ssTorA fused to Δ(1–26)MBP in pTrc99A | This study |
| pssTat–MBP | ssTat‐Δ(1–26)MBP in pBAD18‐Cm where ssTat is any putative E. coliTat signal peptide | Tullman‐Ercek et al. (2007) |
| pssTorA–PhoA | ssTorA‐Δ(1–22)PhoA in pTrc99A | DeLisa et al. (2003) |
| pPhoA | Native PhoA in pTrc99A | This study |
| pΔss‐PhoA | Δ(1–22)PhoA in pTrc99A | This study |
| pssTorA–scFv13.R4 | ssTorA fused to scFv13.R4 in pTrc99A | This study |
| pssMBP–scFv13.R4 | ssMBP fused to scFv13.R4 in pTrc99A | This study |
| pTMM | ssTorA fused to Δ(1–26)MBP with a mini MCS in between the signal and MBP, cloned in pTrc99A | This study |
| pssTorA–POI–MBP | ssTorA signal peptide–POI–Δ(1–26)MBP fusion in pTrc99A | This study |
| pssTorA–MBP–GFP | ssTorA‐Δ(1–26)MBP–GFP fusion in pTrc99A | This study |
| pMBP–GFP | Native MBP fused to GFP in pTrc99A | This study |
| pssTorA–MBP‐scFv13 | ssTorA‐Δ(1–26)MBP‐scFv13 in pTrc99A | This study |
| pssTorA–MBP–scFv13.R4 | ssTorA‐Δ(1–26)MBP–scFv13.R4 in pTrc99A | This study |
| pMBP–scFv13.R4 | Native MBP fused to scFv13.R4 in pTrc99A | This study |