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. 2011 Feb 7;4:1. doi: 10.3389/fnmol.2011.00001

Figure 2.

Figure 2

Reporter gene assays. Kelly cells were transiently transfected (36 h) with reporter gene vectors containing either the ASCL1 promoter sequence or the hASH1 mRNA 5′- and 3′UTRs followed by PMA administration (40 nM). In hASH1 mRNA UTR-dependent reporter assays the firefly-luciferase transcription rate was controlled by the constitutive SV40 promoter. Values of firefly-luciferase activity were normalized to the co-transfected renilla-luciferase. (A) Influence of ASCL1 promoter on PMA-mediated reporter gene activity. (B) hASH1 mRNA 5′- and 3′UTR-dependent response on gene expression rate following PMA treatment. n = 12; *p < 0.05, **p < 0.01, ***p < 0.001.