Figure 4.

Screening for conditions leading to an alteration in gene expression rate mediated by hASH1 mRNA 5′- and 3′UTRs. Kelly cells were transiently transfected (36 h) with reporter constructs where the luciferase mRNA UTRs were replaced by the hASH1 mRNA UTRs. Transcription rate was controlled by the constitutive SV40 promoter. Cells were treated with different compounds for 24 h. The concentrations used were: PMA = 40 nM; Forskolin = 100 nM; adenosine = 100 nM; cobaltous chloride (CoCl2) = 50 nM; 2,2′-Dipyridyl (2,2 DP) = 100 mM; ascorbic acid = 100 mM; dopamine = 100 nM. Data are relative to the relevant control conditions (H2O, ethanol, DMSO respectively). n = 12; **p < 0.01, ***p < 0.001.