TABLE 1.
E. coli strains, phages, and plasmids used in this study
| Strain, phage, or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| Bacterial strains | ||
| BN4020 | fur-1::Tn5 | CGSC no. 7540 |
| BW25113 | lacIqrrnBT14 ΔlacZW116hsdR514 araBADAH33 ΔrhaBADLD78 | B. Wanner |
| RK4353 | Δ(argF-lac) U169 rpsL150 | Laboratory collection |
| YMC18 | endA thi hsdR Δlac rpoN::Tn10 | B. Magasanik |
| JW138 | Δhya Δhyb Φ(hycB′-lacZ) | Laboratory collection |
| SE1174 | fhlA102::Tn10 | Laboratory collection |
| SE1931 | fnr zcj::Tn10 | Laboratory collection |
| SE1265 | pfl-1 zba::Tn10 | Laboratory collection |
| SE1910 | Δ(modE-Km)2 | 18 |
| SE1989 | Δcya-Km CRP* | Laboratory collection |
| SE2147 | moeA113 zbi::Tn10 | 19 |
| VJS720 | modB247::Tn10 | V. Stewart |
| WS219 | RK4353 Δ(hyfB to hyfG)-Cm | This study |
| WS127 | Δ(srl-fhlA) Δlac λWS1-(hycA-lac) | 41 |
| Φ(hyfA′-′lacZ) derivatives | ||
| WS222 | RK4353 λWS4 | This study |
| WS228 | WS222 fhlA::Tn10 | WS222 × (P1)SE1174 |
| WS229 | WS222 rpoN::Tn10 | WS222 × (P1)YMC18 |
| WS230 | WS222 fnr zcj::Tn10 | WS222 × (P1)SE1931 |
| WS231 | WS222 moeA113 | WS222 × (P1)SE2147 |
| WS232 | WS222 Δ(hyfB to hyfG)-Cm | WS222 × (P1)WS219 |
| WS233 | WS222 pfl-1 zba::Tn10 | WS222 × (P1)SE1265 |
| WS235 | WS222 modB247::Tn10 | WS222 × (P1)VJS720 |
| WS236 | WS222 Δ(modE-Km)2 | WS222 × (P1)SE1910 |
| WS266 | RK4353 λWS10 | This study |
| WS267 | WS266 modB247::Tn10 | WS222 × (P1)VJS720 |
| WS268 | WS266 pfl-1 zba::Tn10 | WS266 × (P1)SE1265 |
| WS269 | WS266 rpoN::Tn10 | WS266 × (P1)YMC18 |
| WS270 | WS266 fhlA::Tn10 | WS266 × (P1)SE1174 |
| WS271 | WS266 Δ(modE-Km)2 | WS266 × (P1)SE1910 |
| WS272 | WS266 Δcya-Km | WS266 × (P1)SE1989 |
| WS273 | WS266 fnr zcj::Tn10 | WS266 × (P1)SE1931 |
| WS274 | WS266 moeA113 | WS266 × (P1)SE2147 |
| WS275 | WS266 Δ(hyfB to hyfG)-Cm | WS266 × (P1)WS219 |
| WS280 | WS266 fur::Tn5 | WS266 × (P1)BN4020 |
| AH266 | WS222 Δ(hyfA to hyfJ), Km | This study |
| AH267 | WS222 Δ(hyfR), Km | This study |
| Phages | ||
| P1 | Tn9 Cmrclr-100 | Laboratory collection |
| λRZ5 | λ′bla ′lacZ lacY+ | Laboratory collection |
| λWS4 | λbla+ Φ(hyfA′-lacZ) lacY+ | This study |
| λWS10 | λbla+laclqhyfR+ Φ(hyfA′-lacZ) lacY+ | This study |
| Plasmids | ||
| PKD4 | FRT-kan+-FRT bla+ | B. Wanner |
| pWS2 | pACYC184-fhlA+ | This study |
| pWS132 | pACYC184-fhlA132 | This study |
| pWS165 | pACYC184-fhlA165 | This study |
| pWS42 | pBR322 bcp hyfABCDEFGHIR′ | This study |
| pWS43 | pBR322 nlpB dapA gcvR bcp hyfA′ | This study |
| pWS44 | pBR322 nlpB dapA gcvR bcp hyfA′-lacZ | This study |
| pWTS3 | pWS42 Δhyf-Cm | This study |
| pWTS36 | pBR322-lacIqhyfR+bcp hyfA′-lacZ | This study |
| pWTS37 | pUC19-hyf promoter region | This study |
| pZCam | pZ1918-Cmr | This study |