TABLE 4.
Regulation of HyfR-dependent expression of Φ(hyfA-lacZ) requires cAMP for maximal activation and is repressed in aerobically grown cells
| Strain | Relevant genotypea | β-Galactosidase activityb
|
||
|---|---|---|---|---|
| LBG | LB | LB + cAMP | ||
| WS266 | Wild type | 8,200 | 27,800 | 26,200 |
| WS272 | cya | 5,100 | 3,260 | 39,800 |
| WS266 (aerobic) | Wild type | ND | 180 | ND |
a
All strains are derivatives of WS266, which carries Φ(hyfA-lacZ) hyfR+ via λWS10. Cultures were grown under anaerobic conditions, except where indicated. cAMP, when present, was added at a final concentration of 3 mM.
b
β-Galactosidase activity is expressed as nanomoles·minute−1·(milligram of cell protein)−1. ND, not determined.