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. Author manuscript; available in PMC: 2012 Feb 4.
Published in final edited form as: Mol Cell. 2011 Feb 4;41(3):311–320. doi: 10.1016/j.molcel.2011.01.013

Figure 4.

Figure 4

PAPD1 has activity towards all four nucleotides in vitro. (A). Catalytic activity of PAPD1 towards the four nucleotides, ATP, GTP, CTP and UTP. 10 and 50 ng of the wild-type (WT) enzyme were used, and 50 ng of the D325A mutant were also assayed as a control. (B). Concentration titration of the activity of PAPD1 towards ATP and UTP. The concentrations used for the nucleotides are indicated. (C). The poly(A) and poly(U) polymerase reaction of PAPD1 follows Michaelis-Menten kinetics. The error bars represent ± 1SD.