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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Clin Cancer Res. 2010 Dec 1;16(23):5712–5721. doi: 10.1158/1078-0432.CCR-10-2055

Figure 3.

Figure 3

AlexaFluor 647-labeled C225 demonstrates significant co-localization with the secondary anti-IgG suggesting that the C225-AuNP construct remains intact after internalization into the cell (A). Although there is mild autofluorescence (< 2%) in the far red channel (second column from left, confocal microscopy), using a fluorescent threshold of 7 arbitrary units (a.u.) demonstrates that over 78% of cells contain measurable levels of AF 647 labeled C225 regardless if AuNPs are conjugated (B, based on fluorescence cell counting). Percentages represent the proportion of cells with fluorescence greater than 7 a.u. while the p value compares the mean fluorescence intensity of each distribution to the negative control.