Table 2. Determination of CPE variant copy number in Sporadic PHEO/PGL tumors.

Table 2 shows the expression of CPE variant mRNA copy number in sporadic pheochromocytoma/paraganglioma (PHEO/PGL) samples. Total RNA was extracted from frozen PHEOs/PGLs after homogenization in TRlzol reagent and 0.2μg was then converted to cDNA. A standard curve was set up to determine the CPE mRNA copy numbers by qRTPCR using CPE plasmid construct of eight different concentrations. Copy numbers were calculated using a standard formula. QRTPCR was done on PHEOs/PGLs samples and the crossing point for each sample was plotted on the standard curve to determine the mRNA copy number.

Sample ID	Diagnosis*	Genotype	Copy Numbers
S39	Benign	SPORADIC	175,080
S45	BenignΔ	SPORADIC	6,181,873
S48	Renign	SPORADIC	184,761
S49	Benign	SPORADIC	181,713
S67	Benign	SPORADIC	135,279
S71	Benign*	SPORADIC	6,451,057
S76	Benign&	SPORADIC	7,228,701

^*At the time of surgery

^#Large tumor (8cms)

^ΔThyroid Cancer

^&Capsular and vascular invasion