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. 2004 Jan;186(2):419–426. doi: 10.1128/JB.186.2.419-426.2004

FIG. 4.

FIG. 4.

Analysis of immunobiological activity of the recombinant NAD+ synthetase on mouse lymphocytes by flow cytometry. (A) Typical expression of CD69 on the surfaces of splenic B (IgM+) or T (CD3+) lymphocytes after incubation of total spleen cells with RPMI (control) or after treatment with 50 μg of recombinant NAD+ synthetase. (B) Percentages of T (CD3+) and B (IgM+) lymphocytes expressing CD69 6 h after in vitro stimulation with recombinant NAD+ synthetase. The results represent means ± standard deviations of triplicate samples repeated twice.