TABLE 1.
Expression of CD69 on mouse B lymphocytes analyzed by flow cytometry
| B-cell marker | % CD69+ cellsa
|
|
|---|---|---|
| RPMI | NAD+ synthetase | |
| CD19 | 11.2 ± 0.3 | 72.1 ± 2.6 |
| B220 | 10.4 ± 0.8 | 65.0 ± 4.5 |
| CD21 | 12.4 ± 1.4 | 70.1 ± 3.7 |
| IgM | 10.4 ± 0.8 | 68.4 ± 3.7 |
a
Percentage of the CD19+-, B220+-, CD21+-, and IgM+-B-cell populations expressing CD69 after incubation of total spleen cells with RPMI (control) or after stimulation with 50 μg of recombinant NAD+ synthetase. The results represent the means and standard deviations of triplicate samples repeated twice.