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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Cancer Res. 2010 Dec 15;70(24):10150–10160. doi: 10.1158/0008-5472.CAN-10-1852

Figure 4.

Figure 4

Vaccination stimulated functional antibodies to angiopoietin-1 and -2. (A). Longitudinal analysis of metastatic melanoma patient M30 (sera 1:100). Day 0 is pre-vaccination. (B). Longitudinal analysis of metastatic non-small cell lung carcinoma patient L19. Day 0 is pre-vaccination. (C). Post-vaccination sera from M30 specifically blocks angiopoietin-1 and -2 induced tube formation. Serum was absorbed against recombinant cytokine as indicated. This experiment was performed twice with similar results. Tube forming scoring (average of two replicates): Pre-VAX (angiopoietin-1 and -2) 3.5, post-VAX (angiopoietin-1 and -2) 0, post-VAX absorbed (angiopoietin-1 and -2) 1.5. (D). Post-vaccination sera from M30 and L19 specifically block angiopoietin-2 stimulated migration of Tie-2 expressing monocytes. Cell migration in response to cytokine as compared to media is depicted. Serum was absorbed against recombinant cytokine as indicated. Similar results were observed in a second experiment.