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Angiopoetin-1 and -2 trigger ERK phosphorylation. (A). HUVECs were treated with 200 ng/ml of angiopoietin-1 or 500 ng/ml angiopoietin-2 for varying times. Cell lysates were analyzed for phospho-ERK with immunoblotting. (B). HUVECs were treated for ten minutes with varying concentrations of angiopoietin-1 and -2, and then phospho-ERK was assayed with immunoblotting. (C). Phospho-ERK levels from (B) were analyzed with an ELISA.
