Skip to main content
. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Cancer Res. 2010 Dec 15;70(24):10150–10160. doi: 10.1158/0008-5472.CAN-10-1852

Figure 6.

Figure 6

Vaccine induced antibodies block angiopioetin-1/2 signaling. HUVECs were treated for ten minutes with 50 ng/ml of angiopoietin-1 or 200 ng/ml of angiopoietin-2 in the presence of PBS or 1 mg/ml of purified immunoglobulins from pre- or post-vaccination sera (with and without absorption with recombinant cytokine). Cell lysates were assayed for phospho-ERK with an ELISA. (A). Stage IV melanoma patient M30. (B). Stage IV lung carcinoma patient L19. Similar results were observed in two experiments.