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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Clin Cancer Res. 2010 Nov 23;17(3):427–436. doi: 10.1158/1078-0432.CCR-09-3069

Figure 2.

Figure 2

Downregulation of HtrA1 in Hec1A and 1B cells promoted invasion. (A and B) Results from matrigel invasion assay show increased number of Hec1A and Hec1B cells invading through the basement membrane (expressed as % of control cells) in scr control compared to HtrA1siRNA transfected cells respectively. There was minimal effect on the migratory capacity. Insets in (A and B) shows efficient downregulation of HtrA1 with HtrA1 siRNA compared to scr control by immunoblot analysis with β-actin loading control (lower panel) in Hec1A and Hec1B cells respectively. NS- Not significant, **P =<0.01 and *P=<0.05 between HtrA1 siRNA and scr control. (C and D) Results from scratch assay of Hec1A and Hec1B with two different shRNAs against HtrA1. The insets in C and D show the levels of stable downregulation of HtrA1 In these cells.