Skip to main content
. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Cancer Res. 2010 Nov 2;70(22):9463–9472. doi: 10.1158/0008-5472.CAN-10-2388

Figure 2. LIN28 plays a functional role in the maintenance of ALDH1+ tumor cells.

Figure 2

A. LIN28 was knocked down by two independent lentiviral shRNA clones in two LIN28+ tumor cell lines (A2780 and T47D). Reduced protein expression of LIN28 was confirmed by Western blots. Full-length blots are presented in Supplemental Figure S1. B. The percentage of ALDH1br tumor cells in the above cell lines was measured using the ALDEFLUOR assay. Blocking endogenous LIN28 expression by shRNAs significantly reduced the percentage of ALDH1br tumor cells. C. LIN28 was ectopically expressed in MCF7 cells by lentiviral infection. Enforced protein expression of LIN28 was confirmed by Western blots. D. The percentage of ALDH1br tumor cells in the above cell lines was measured using the ALDEFLUOR assay. Enforced LIN28 expression significantly increased the percentage of ALDH1br tumor cells. E. Typical morphology of mammospheres in MCF7 cells. F. Enforced LIN28 expression significantly increased mammosphere numbers compared to the control cells. Left panel presents the result in the first generation of mammospheres; right panel presents the result in the third generation of mammospheres. All p-values <0.05.