FIG. 5.

Northern hybridization analysis of S. aureus 8325-4 and SH1000 trxA and trxB transcripts: effect of oxidative stress agents. Cultures of 8325-4 and SH1000 were grown aerobically to the mid-exponential phase of growth and treated with (A) menadione, (B) τ-butyl hydroperoxide, and (C) hydrogen peroxide at the concentrations and for the times shown. Total RNA obtained from cultures at an OD₆₀₀ of ≈1.0 was electrophoresed, blotted, and hybridized to trxA and trxB probes (see Materials and Methods). Arrows indicate the positions of transcripts.