TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristicsa | Source or reference |
|---|---|---|
| E. coli XL-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqlacZΔM15 Tn10(Tcr)] | Laboratory stock |
| S. aureus | ||
| Oxford (NCTC6571) | Wild-type laboratory strain | Laboratory stock |
| RN4220 | Restriction-deficient mutant of 8325-4 (UV cured of prophages) used as primary recipient for plasmids propagated in E. coli; spontaneous mutant with 11-bp deletion in rsbU | 12, 24 |
| SH1000 | Derivative of 8325-4 carrying intact rsbU gene | Gift of S. Foster |
| Plasmids | ||
| pAUL-A | Temperature-sensitive E. coli-S. aureus shuttle vector, lacZ Emr | 45 |
| pO17 | pAUL-A containing ∼0.7-kbp internal S. aureus Oxford trxB DNA fragment | This study |
| pO18 | pAUL-A containing ∼0.7-kbp internal S. aureus Oxford glnA DNA fragment | This study |
| pO115 | pAUL-A containing internal S. aureus trxB and glnA DNA fragments in tandem | This study |
a
Abbreviations: Emr, and Tcr, resistance to erythromycin/lincomycin and tetracycline, respectively.